THE EFFECT OF SOLVENT TYPES ON ANTIOXIDANT ACTIVITY OF AVOCADO LEAF EXTRACT (Persea americana Mill)
Article Sidebar
Main Article Content
Abstract
Avocado plant (Persea americana Mill) is one of the plants that have the benefit of preventing premature aging due to the antioxidant content. This study aims to determine the effect of solvent type ethanol 70%, ethyl acetate, n-hexane on antioxidant activity of avocado leaf extract (Persea americana Mill). The method used in this study was laboratory experimental, using 3 types of solvents in avocado leaf extract (Persea americana Mill) with several concentrations of 50 ppm, 100 ppm, 150 ppm, 200 ppm, and 250 ppm. Antioxidant activity of avocado leaves (Persea americana Mill) was conducted using the DPPH method, by analyzing the activity of free radical immersion in avocado leaf extract which was determined based on the value of IC50, then the data obtained were tested statistically. The results showed that the type of ethanol extract solvent has a very strong antioxidant activity with a value of IC50 24.69 ppm; the type of ethyl acetate extract solvent has a weak antioxidant activity with a value of IC50 172.84 ppm; and the solvent type of n-hexane extract has very weak antioxidant activity with IC50 value of 41.22 ppm. Different types of solvents affect the antioxidant activity of avocado leaf extract (Persea americana Mill).
Article Details
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
References
Alamsyah, H. K., Widowati, I., & Sabdono, A. 2014. Aktivitas antibakteri ekstrak rumput laut sargassum cinereum (jg agardh) dari perairan pulau panjang jepara terhadap bakteri escherichia coli dan staphylococcus epidermidis. Journal of Marine Research, 3(2), 69-78.
Handayani, S., Najib, A. & Wati, N. P . 2018. Uji Aktivitas Antioksidan Ekstrak Daun Daruju (Acanthus ilicifolius L.) dengan Metode Peredaman Radikal Bebas 1,1- diphenyil-2-picrylhidrazil (DPPH). Jurnal Fitofarmaka Indonesia 2018, 5(2), 299– 308
Husni, P. & Pratiwi, S., (2017). Artikel tinjauan: potensi penggunaan fitokonstituen tanaman Indonesia sebagai bahan aktif tabir surya. Jurnal Farmaka, 15(4), 18-25.
Kemenkes RI. (2017). Farmakope Herbal Indonesia edisi II. Jakarta: Kementrian Kesehatan Republik Indonesia.
Kinam, B. O. I., Prabowo, W. C., Supriatno, S., & Rusli, R. (2021). Skrining Fitokimia dan Profil KLT Ekstrak dan Fraksi dari Daun Berenuk (Cresentia cujete L.) serta Uji DPPH: Phytochemical Screening and TLC Profile of Extracts and Fractions from Leaves of Berenuk (Cresentia cujete L.) and DPPH Test. In Proceeding of Mulawarman Pharmaceuticals Conferences (Vol. 14, pp. 339-347).
Kusuma, I. M., Veryanti, P. R., & Chairunnisa, B. (2020). Aktivitas Antioksidan dari Ekstrak Metanol Buah Kawista (Limonia acidissima) dengan Metode DPPH (1, 1-difenil-2-pikrilhidrazil). jurnal Sainstech Farma, 13(2), 60-65
Maliana, D., Nuryanti, N., & Harwoko, H. (2016). Formulasi sediaan krim antioksidan ekstrak etanolik daun Alpukat (Persea americana Mill.). Acta Pharmaciae Indonesia, 4(2), 7-15.
Malik, A., Edward, F., & Waris, R. (2014). Skrining fitokimia dan penetapan kandungan flavonoid total ekstrak metanolik herba boroco (Celosia argentea L.). Jurnal Fitofarmaka Indonesia, 1(1), 1-5.
Nathania, E. K., Maarisit, W., Potalang, N. O., & Tapehe, Y. (2020). Uji Aktivitas Antioksidan Ekstrak Etanol Daun Kecubung Hutan (Brugmansia Suaveolens Bercht. & J. Presl) Dengan Menggunakan Metode DPPH (1,1-diphenyl02-picrylhydrazil). Jurnal Biofarmasetikal Tropis, 3, 40-47.
Noviyanti, Y. Hepiyansori, Marlina, R. 2019. Identifikasi senyawa flavonoid dari ekstrak etanol kulit jeruk kalamansi (Citrus microcarpa). Jurnal Ilmiah Farmasi, 6(2), 312-321.
Nurulita, N. A. dkk. (2019) „Uji Aktivitas Antioksidan dan Anti Aging Body Butter dengan Bahan Aktif Ekstrak Daun Kelor‟, Jurnal Ilmu Kefarmasian Indonesia, 17(1), p. 1. doi: 10.35814/jifi.v17i1.543.
Pamungkas, D. K., Retnaningtyas, Y., & Wulandari, L. (2017). Pengujian Aktivitas Antioksidan Kombinasi Ekstrak Metanol Daun Mangga Gadung (Mangifera indica L. var. gadung) dan Ekstrak Etanol Daun Pandan Wangi (Pandanus amaryllifolius Roxb.). Pustaka Kesehatan, 5(1), 46-49.
Prananda, Y. (2015). Skrining fitokimia ekstrak etanol daun simpur (Dillenia indica l.) sebagai tahapan awal pada pengujian toksisitas. Jurnal Mahasiswa Farmasi Fakultas Kedokteran UNTAN, 3(1).
Rizkayanti, Diah, A.W.M. and Jura, M.R. 2017. Uji Aktivitas Antioksidan Ekstrak Air dan Ekstrak Etanol Daun Kelor (Moringa oleifera LAM). Jurnal. Akad. Kim. 6. (2): 125-131
Sari, Ayu Nirmala, 2015. "Antioksidan alternatif untuk menangkal bahaya radikal bebas pada kulit." Elkawnie: Journal of Islamic Science and Technology 1.1 (2015): 63-68.
Simaremare, E. S. (2014). Skrining fitokimia ekstrak etanol daun gatal (Laportea decumana (Roxb.) Wedd). PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia), 11(1).
Widarta, I. W. R., & Wiadnyani, A. A. I. S. (2019). Pengaruh metode pengeringan terhadap aktivitas antioksidan daun alpukat. Jurnal Aplikasi Teknologi Pangan, 8(3), 80-85.
Yanty, Y. N., Sopianti, D. S., & Veronica, C. (2019). Fraksinasi dan skrining fraksi biji kebiul (Caesalpinia bonduc (L) ROXB) dengan metode KLT (kromatografi lapis tipis) fraction and screening of fresh seed (Caesalpinia bonduc (L) Roxb Seeds With KLT Method (Thin Lapic Chromatography). Borneo Journal of Pharmascientech, 3(1).