ANTIBACTERIAL POTENCY OF Cajanus cajan to Staphylococcus epidermidis
Keywords:
Cajanus sajan, antibacterial, Staphylococcus epidermidis, inhibition zoneAbstract
One legume plant suspected of containing flavonoids such as quercetin, luteolin, and kajanol is the pigeon pea plant. Due to its flavonoid content, pigeon pea plants have antibacterial, anti-inflammatory, antiviral, and antitumor properties. This study aimed to identify the antibacterial compounds found in pigeon pea plants and determine their antibacterial activity, as measured by the inhibition zones of various pigeon pea extracts against Staphylococcus epidermidis.
This study was pre-experimental, using a maceration extraction method with 96% ethanol, n-hexane, and ethyl acetate solvents. The extracts tested for antibacterial activity were ethanol extract, n-hexane extract, and ethyl acetate extract. Antibacterial activity was observed from the inhibition zones on semisolid agar media, indicated by the clear zone.
The results showed that the antibacterial compounds contained in pigeon pea extracts include flavonoids, alkaloids, saponins, tannins, polyphenols, and steroids. In the bacterial inhibition zone test, the 96% ethanol extract had a more dominant inhibition zone than the n-hexane and ethyl acetate extracts. This can be observed through the inhibition zone ranges in 96% ethanol of 16.2±0.475 mm, ethyl acetate of 15.2±0.757 mm, and n-hexane solution of 14.4±0.321 mm. The effectiveness range for the inhibition zone against Staphylococcus epidermis bacteria is in the low to moderate range.
References
1. Hano. 2020. Bioaktivitas Asam jawa (Tamarindus indica) dan Pemanfaatannya. Flora : Jurnal Biologi dan Pembelajarannya, 7(2), 85.
2. Agus, Sarifa; Achmadi, Suminar Setiati; dan Mubarik, Nisa Rachmania. 2017. Antibacterial activity of naringenin-rich fraction of pigeon pea leaves toward Salmonella thypi. Asian Pac J Trop Biomed. 7(8): 725–728.
3. Fauzi, M, Rizal; Bintari, Yoni, Rina; dan Damayanti, Dini, Sri. 2023.. Potensi Penghambatan Pertubuhan Escherichia coli dan Salmonella typhi Dari Tempe Kedelai, Tempe Kacang Merah, dan Tempe Kacang Tanah. UNISMA. Vol. 1. No. 1. Hal. 1-7.
4. Pujiati; dan Primiani, Cicilia, Novi. 2016. Uji Antibakteri Kacang Gude (Cajanus cajan) Terhadap Bakteri Staphylococcus aureus dan Escerichia coli. Prosiding Seminar Nasional Biodiversitas VI. Hal. 659-665.
5. Wulandari¸Sri; Aryati, Fika; Ramadhan, Adam, M; dkk. 2021. Sintesis Senyawa Phenyllactic Acid (2-Hydroxy-3-Phenylpropionic acid) dan Aktivitasnya Sebagai Antibakteri. Jamb.J.Chem. 3 (2), 91-98.
6. Hartati, Indah. 2012. Pemurnan Enzim Selulase Dari Rumen Sapi Menggunakan Teknologi Expanded Bed Adsorption. Techno. Vol. 13. No. 1. Hal. 43 - 51.
7. Ningsih, D.R. Zusfahair, dan Mantari, D. 2017. Ekstrak Daun Mangga (Mangifera indica L.) Sebagai Antijamur Terhadap Jamur Candida albicans Dan Identifikasi Golongan Senyawanya. Jurnal Kimia Riset, Volume 2 No. 1, Juni 2017.Jurusan Kimia Fmipa Universitas Jenderal Soedirman Purwokerto.
8. Julianti, Reska., Harlis, Harizon. 2017. Pengaruh Ekstrak Daun Manggis (Garcinia mangostana Linn.) Terhadap Pertumbuhan Bakteri Escherichia coli Sebagai Pengayaan Bahan Ajar Praktikum Mikrobiologi. Jambi: Pendidikan Biologi Jurusan PMIPA FKIP.
9. Magvirah¸ Tiara; Marwati; dan Ardhani, Fikri. 2019. Uji Daya Hambat Bakteri Staphylococcus aureus Menggunakan Ekstrak Daun Tahongai (Kleinhovia hospita L.). Jurnal Peternakan Lingkungan Tropis. Vol. 2. No. 2. Hal. 41-50.
10. Hastuti, Luthfiana Indah; dan Retnaningrum, Endah. 2020. Kemampuan Fermentasi Bal Dengan Substrat Susu Kacang Merah. Bioeksperimen. Vol. 6. No. 2. Hal. 116-122.
11. Sine, Yuni; dan Pardosi, Lukas. 2021. Perubahan Kandungan Antioksidan Kacang Gude (Cajanus cajan (L) Millsp.) pada Proses Fermentasi Tempe Gude. Jurnal Pendidikan Biologi Undiksha. Vo. 8. No. 1. Hal. 1-6.
12. Yuni. 2021. Perubahan Kandungan Antioksidan Kacang Gude (Cajanua cajan L. Millsp) Pada Proses fermentasi tempe Gude. Jurnal Pendidikan Biologi Undiksha,. 8(1), pp. 2599-1450.
13. Radji, M. 2011, Buku Ajar Mikrobiologi Panduan Mahasiswa Farmasi dan Kedokteran. Jakarta: EGC.
14. Talari, Aruna; dan Shakappa, Devindra. 2018. Role of pigeon pea (Cajanus cajan L.) in human nutrition and health: A review. Asian J. Dairy & Food Res. 37(3): 212-220.
15. Rahayu. 2017. Aktivitas Antibakteri Ekstrak Etanolik Daun Juwet (Syzygium cumini L. Skeels) Terhadap Bakteri isolate Klinis Salmonella typhi, Pharmacy : Jurnal Farmasi Indonesia, 17(1), 138.
16. Puspodewi. 2015. Daya Hambat Daun Asam Jawa (Tamarindus indica) Terhadap Pertumbuhan Salmonella typhi Penyebab Demam Tifoid. The 2nd University Research Coloquium 2015, 45-50
17. Mukhriani. 2014. Ekstrak Pemisahan Senyawa dan Identifikasi Sediaan Aktif. Jurnal of Pharmacy, VII(2), 361.
18. Moensaku, Elmalinda; Sine, Yuni; dan Pardosi, Lukas. 2021. Isolasi dan identifikasi kapang Rhizopus pada tempe kacang merah (Phaseulus vulgaris L). Jurnal Pendidikan Biologi Undiksha. Vol. 8. No. 2. Hal. 61-69
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